PanProbes™ Universal qPCR MasterMix
The PanProbes™ Universal qPCR MasterMix is a ready-to-use, 2x concentrated master mix optimized for probe-based real-time PCR. Designed for compatibility with a wide range of commercial qPCR systems (both ROX-dependent and ROX-independent), this master mix ensures reliable and accurate results every time.
- Enhanced Antibody-Mediated Hot-Start Taq DNA Polymerase: Ensures minimal non-specific amplification, increasing the specificity and efficiency of your reactions.
- Optimized for Probe-Based Real-Time PCR: Achieve accurate quantification with high sensitivity.
- Versatile Compatibility: Works with most commercially available qPCR systems, offering flexibility in your experimental setup.
- Comprehensive Reaction Components: Contains dNTPs, MgCl2, stabilizers, and enhancers—everything you need for a successful PCR reaction.
This PanProbes™ Universal qPCR MasterMix simplifies the workflow while enhancing PCR performance, making it an essential tool for researchers seeking reproducibility and high-quality results.
Figure 1. Performance of PanProbes™ Universal qPCR MasterMix
- Combine the assay Master Mix thoroughly to ensure consistency and equally dispense the solution into each qPCR tube or into the wells of a qPCR plate. Employ good pipetting practice to ensure assay precision and accuracy.
- Add DNA samples (and DNase-free H2O if needed) to the PCR tubes or wells containing assay Master Mix (Table 1), seal the tubes or wells with flat caps or optically transparent film. Note: to ensure thorough mixing of reaction components, vortex for approximately 30 seconds (or more).
- Spin the tubes or plate to remove any air bubbles and collect the reaction mixture in the vessel bottom.
- Setup the thermal cycling protocol on a real-time PCR instrument according to Table 2. Note: optimization may be needed for better performance.
- Load the PCR tubes or plate into the real-time PCR instrument and commence the run.
- Perform data analysis according to the instrument-specific instructions.
Note: Optimal conditions for amplification will vary depending on the primers and thermal cycler used. It may be necessary to optimize the system for individual primers, template, and thermal cycler.
Template
- Purified high quality DNA is needed for a success PCR reaction.
- The final concentration of DNA template please refer to table 1.
Important notes
- Shake gently before use to avoid foaming and low-speed centrifugation.
- During operation, always wear a lab coat, disposable gloves, and protective equipment