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UltraScenceCLIA FemtoPlus Western Substrate

The UltraScenceCLIA FemtoPlus Western Substrate is a highly sensitive chemiluminescent substrate designed for use in Western blotting applications. Engineered to provide enhanced signal amplification, this substrate is perfect for detecting low-abundance proteins in immunoblotting experiments, making it ideal for both basic research and clinical diagnostics.

 Product Description

- Chemiluminescence Immunoassay (CLIA)

Chemiluminescence Immunoassay (CLIA) is a highly sensitive and specific technique that combines chemiluminescence with immunochemical reactions to detect and analyze a wide range of substances such as antigens, antibodies, and enzymes. Due to its high sensitivity, safety, and specificity, CLIA has gained significant popularity in fields like clinical diagnosis, environmental monitoring, and pharmaceutical analysis.

                                                                                                               

Chemiluminescence immunoassay (CLIA) encompasses several methods based on different luminescence mechanisms, including direct chemiluminescence immunoassay, chemiluminescence enzyme immunoassay (CLEIA), and electrochemiluminescence immunoassay. In the chemiluminescence enzyme immunoassay, an enzyme—commonly horseradish peroxidase (HRP)—is conjugated to an antigen or antibody. Following the immunoreaction, the substrate luminol emits light through an oxidation-reduction reaction catalyzed by the enzyme-labeled immune complex and luminescent reagents. The amount of antigen or antibody is then quantified by measuring the intensity of the emitted light.

Although this reaction produces rapid light emission, the luminescence duration is relatively short, which can compromise the stability and reproducibility of detection results. Research has demonstrated that the addition of one or more chemical enhancers can significantly boost the luminescent intensity and extend the duration of light emission.

Our specially formulated reagent not only effectively amplifies the luminescent signal but also substantially prolongs the luminescence time, resulting in more stable and reliable assay outcomes.

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Performance

Figure 1. Evaluation of Hepatitis B antigen (HBsAg) clinical samples using chemiluminescence immunoassay (CLIA). A total of 24 clinical samples were analyzed for both qualitative and quantitative determination of HBsAg. The results were directly compared with a competitor assay. Testing was performed using the Dia. Pro instrument, SARA, demonstrating the assay’s accuracy and reliability in clinical diagnostics.

                                                                                                    

                                                                                                                                    

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Figure 2. Assessment of Hepatitis C antigen (HCV Ag) in clinical samples using chemiluminescence immunoassay (CLIA). Eight clinical samples were tested for both qualitative and quantitative analysis, with results compared directly against a competitor assay. The assay was conducted on the Dia. Pro instrument, SARA, highlighting its precision and effectiveness in clinical diagnostics.

                                                                                    

                                                 

                                                                                                                            

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Figure 3. Evaluation of HIV antibody/antigen (HIV Ab/Ag) in clinical samples using chemiluminescence immunoassay (CLIA). Thirteen clinical samples were analyzed for both qualitative and quantitative determination, with results compared side-by-side to a competitor assay. The assay was performed on the Dia. Pro instrument, SARA, demonstrating high accuracy and reliability for clinical diagnostic applications.

                                                                             

               

                                                                                                                                

Why Choose UltraScenceCLIA FemtoPlus ?

  • Ultra-High Sensitivity : Provides extremely sensitive chemiluminescent detection, allowing researchers to visualize proteins with picogram-level detection sensitivity.
  • Superior Signal Strength : Offers stronger, longer-lasting signals compared to standard substrates, minimizing the need for repeated exposures in Western blot assays.
  • Quick and Easy Application : Simplifies the workflow by enabling rapid development and clear, high-quality results with minimal background noise.
  • Optimized for Low-Abundance Proteins : Perfect for low-abundance protein detection, making it an essential tool in biomarker research, disease diagnosis, and protein quantification studies.

 Key Features of UltraScenceCLIA FemtoPlus Western Substrate ! 

  • High Sensitivity : Detects even the most low-abundant proteins, providing exceptional sensitivity for Western blot applications.
  • Chemiluminescent Detection : Utilizes a chemiluminescence-based detection method that enables visualizing protein bands with high clarity and minimal background interference.
  • Long Signal Duration : The signal lasts significantly longer than traditional substrates, providing ample time for image capture and reducing experimental variability.
  • Ready-to-Use : The substrate is provided in a ready-to-use format, eliminating the need for complex preparation and ensuring quick application.
  • Compatible with Standard Detection Systems : Works seamlessly with chemiluminescent imaging systems, ensuring compatibility with most Western blotting protocols.

UltraScence Femto Plus Western Substrate | 50 ml x 2 / Set

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