EMBER™ Ultra RNA Gel System is an advanced electrophoresis
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The EMBER™ Ultra RNA Gel System is an advanced electrophoresis platform designed for high-resolution RNA analysis. It provides superior separation and detection of RNA molecules, making it ideal for assessing RNA integrity, purity, and size distribution.
The system employs optimized gel formulations to minimize RNA degradation and improve band sharpness, ensuring accurate results for downstream applications such as qPCR, RNA sequencing, and northern blotting.
Its compatibility with fluorescence-based detection enhances sensitivity, making it a powerful tool for RNA quality control in molecular biology research.
The EMBER™ Ultra RNA Gel System Protocol
Materials Required
EMBER™ Ultra RNA Gel
RNA sample (purified and quantified)
RNA Loading Buffer
RNA Ladder (for size reference)
Gel Electrophoresis System
Electrophoresis Buffer (provided with the system or recommended by the manufacturer)
Agarose Gel Running Tray
UV or Blue Light Transilluminator
Protocol Steps
1. Preparation of Gel
Unpack the EMBER™ Ultra RNA Gel
Ensure the gel is at room temperature before use.
If the gel is pre-cast, remove the protective film.
Prepare the Electrophoresis Buffer
Use the recommended running buffer (often a MOPS or TBE-based buffer).
Fill the electrophoresis chamber with sufficient buffer to submerge the gel.
2. RNA Sample Preparation
Prepare RNA Samples
Dilute RNA in RNase-free water if necessary.
Add the RNA Loading Buffer in a 1:1 ratio.
Heat the mixture at 70°C for 5 minutes to denature secondary structures.
Quickly chill on ice to prevent renaturation.
Prepare the RNA Ladder
Mix the RNA ladder with the RNA Loading Buffer.
Heat at 70°C for 5 minutes, then cool on ice.
3. Gel Loading and Electrophoresis
Load the Gel
Carefully load RNA samples and ladder into the wells using a micropipette.
Avoid overloading to prevent lane distortion.
Run the Electrophoresis
Set the voltage to 100–120V.
Run for 30–45 minutes, or until the dye front reaches 75% of the gel length.
4. RNA Visualization and Analysis
Visualize the Gel
Place the gel on a UV or Blue Light Transilluminator.
Use an RNA-safe dye if the gel is unstained.
Interpret Results
Compare RNA bands to the ladder to determine sample integrity and size.
Look for clear 28S and 18S rRNA bands (for eukaryotic RNA).
5. Post-Electrophoresis Processing
Image Documentation
Capture gel images using a gel documentation system.
Adjust brightness/contrast as needed for clarity.
Dispose of Used Materials
Follow laboratory guidelines for gel and buffer disposal.
This protocol ensures high-resolution RNA analysis using the EMBER™ Ultra RNA Gel System.