Rejoignez-nous chez Gentaur Bruxelles pour cet événement de 2 heures

Chaque année, nous invitons notre communauté, nos laboratoires et nos utilisateurs à venir rencontrer Gentaur notre distributeur en Europe! C'est l'événement idéal pour se retrouver et présenter les nouvelles techniques telles que EMBER™ Ultra RNA Gel System. 

The EMBER™ Ultra RNA Gel System is an advanced electrophoresis platform designed for high-resolution RNA analysis. It provides superior separation and detection of RNA molecules, making it ideal for assessing RNA integrity, purity, and size distribution.

 The system employs optimized gel formulations to minimize RNA degradation and improve band sharpness, ensuring accurate results for downstream applications such as qPCR, RNA sequencing, and northern blotting. 

Its compatibility with fluorescence-based detection enhances sensitivity, making it a powerful tool for RNA quality control in molecular biology research. 

The EMBER™ Ultra RNA Gel System Protocol

Materials Required

EMBER™ Ultra RNA Gel

RNA sample (purified and quantified)

RNA Loading Buffer

RNA Ladder (for size reference)

Gel Electrophoresis System

Electrophoresis Buffer (provided with the system or recommended by the manufacturer)

Agarose Gel Running Tray

UV or Blue Light Transilluminator

Protocol Steps

1. Preparation of Gel

Unpack the EMBER™ Ultra RNA Gel

Ensure the gel is at room temperature before use.

If the gel is pre-cast, remove the protective film.

Prepare the Electrophoresis Buffer

Use the recommended running buffer (often a MOPS or TBE-based buffer).

Fill the electrophoresis chamber with sufficient buffer to submerge the gel.

2. RNA Sample Preparation

Prepare RNA Samples

Dilute RNA in RNase-free water if necessary.

Add the RNA Loading Buffer in a 1:1 ratio.

Heat the mixture at 70°C for 5 minutes to denature secondary structures.

Quickly chill on ice to prevent renaturation.

Prepare the RNA Ladder

Mix the RNA ladder with the RNA Loading Buffer.

Heat at 70°C for 5 minutes, then cool on ice.

3. Gel Loading and Electrophoresis

Load the Gel

Carefully load RNA samples and ladder into the wells using a micropipette.

Avoid overloading to prevent lane distortion.

Run the Electrophoresis

Set the voltage to 100–120V.

Run for 30–45 minutes, or until the dye front reaches 75% of the gel length.

4. RNA Visualization and Analysis

Visualize the Gel

Place the gel on a UV or Blue Light Transilluminator.

Use an RNA-safe dye if the gel is unstained.

Interpret Results

Compare RNA bands to the ladder to determine sample integrity and size.

Look for clear 28S and 18S rRNA bands (for eukaryotic RNA).

5. Post-Electrophoresis Processing

Image Documentation

Capture gel images using a gel documentation system.

Adjust brightness/contrast as needed for clarity.

Dispose of Used Materials

Follow laboratory guidelines for gel and buffer disposal.

This protocol ensures high-resolution RNA analysis using the EMBER™ Ultra RNA Gel System.