In the rapidly evolving world of single-cell analytics and live cell research, accurate, high-resolution monitoring systems are essential for understanding complex biological processes in real time. ATTO provides a suite of powerful tools designed for live cell research that enable scientists to monitor gene expression, cellular behavior, and other biological phenomena over time. Among these systems are Cellgraph, CytoWatcherII, Kronos Dio, and Kronos HT, each offering unique features to meet the diverse needs of research labs.
1. Cellgraph: Real-Time Monitoring for Cellular Behavior
Cellgraph is a versatile system developed for high-resolution imaging of live cells. It allows researchers to visualize cellular dynamics in real-time, making it ideal for studying cell migration, morphology, and cell-cell interactions.
Key Features:
- High-Resolution Imaging: Cellgraph delivers high-quality images, providing real-time data on cellular processes.
- Versatile Applications: It is perfect for observing various cellular behaviors, such as cell proliferation, apoptosis, and differentiation.
- Live-Cell Imaging: The system maintains optimal conditions for live cells, including temperature control, CO2 regulation, and humidity control, ensuring that cellular behavior is observed under physiological conditions.
Applications:
- Monitoring cell migration and adhesion in wound healing assays.
- Observing cell proliferation and drug response.
- Investigating cellular stress and differentiation pathways.
Activation of GnRH neurons by kisspeptin stimulation: Ultradian rhythms of gonadotropin-releasing hormone (GnRH) gene transcription in single GnRH neurons were monitored using cultured hypothalamic slice prepared from transgenic mouse expressing a GnRH promoter-driven destabilized liciferase reporter. It was demonstrated that GnRH gene transcription was synchronously activated after kisspeptin was added to the media. Reference: HK. Choe, Proc. Natl. Acad. Sci. U.S.A., 110(14), 5677-5682 (2013) [PubMed]
Visualization of ATP oscillations in the early stage of chondrogenesis: Cellular condensation in embryonic limbs that occurs in the early stage of chondrogenesis is considered to play a critical role in the secretion of adhesion molecules and extracellular matrixes. The movie shows the visualization of ATP oscillation after the induction of chondrogenesis in ATCD5 cells transfected with an ATP-dependent Phyxothrix hirtus luciferase gene. As demonstrated in this study, the Cellgraph system is an effective tool for examining intracellular metabolic mechanisms. Reference: H. Kwon, Cell Death Disease, 3. e278 (2012) [PubMed]
2. CytoWatcherII: Comprehensive Live-Cell Tracking
The CytoWatcherII system offers a comprehensive solution for real-time monitoring of cellular activity, providing users with a high degree of precision and sensitivity. This system is designed to observe cellular changes in a variety of applications, from cellular stress assays to detailed gene expression studies.
Key Features:
- Non-Invasive Monitoring: CytoWatcherII utilizes advanced imaging technology to track live cells without disturbing their natural environment.
- Long-Term Monitoring: It allows for extended monitoring periods, enabling the observation of dynamic cellular processes over days or even weeks.
- Flexible Imaging Modes: CytoWatcherII can track both fluorescence and bioluminescence, allowing users to visualize multiple cellular events simultaneously.
Applications:
- Long-term monitoring of cellular responses to drug treatments.
- Real-time tracking of protein expression and cell signaling.
- Studying autophagy and mitochondrial function in live cells.
3. Kronos Dio: Real-Time Luciferase Assay for Gene Expression
The Kronos Dio system takes real-time gene expression analysis to the next level with its innovative luciferase assay capabilities. It is designed to monitor gene transcription in real-time using bioluminescence, making it a valuable tool for studying gene regulation, drug responses, and more.
Key Features:
- Real-Time Gene Expression Monitoring: The Kronos Dio system provides continuous, real-time monitoring of luciferase activity, allowing researchers to track gene expression as it happens.
- High Sensitivity: With enhanced sensitivity, Kronos Dio is ideal for detecting even low levels of gene expression, making it suitable for both gene transfer efficiency and drug response studies.
- Multicolor Capability: This system supports multicolor luciferase assays, enabling the analysis of multiple genes or pathways simultaneously.
Applications:
- Real-time monitoring of gene expression in response to stimuli or drug treatment.
- Studying transcription factor activity and genetic regulation.
- Analyzing biological clock genes and circadian rhythm in live cells.
4. Kronos HT: High-Throughput, High-Sensitivity Gene Expression Monitoring
The Kronos HT system is ATTO’s high-throughput solution for real-time gene expression monitoring. It is designed to handle high-volume assays without compromising on sensitivity or accuracy, making it perfect for large-scale experiments and screenings.
Key Features:
- High-Throughput Design: With the ability to handle up to 192 samples (using 96-well plates), Kronos HT is ideal for large-scale screening applications.
- Real-Time Data Collection: The system provides continuous monitoring of bioluminescence over time, allowing for tracking of gene expression dynamics.
- Cell-Friendly Operation: Kronos HT features a non-vibrating scanning mechanism, ensuring that delicate live cells are not disturbed during measurement.
Applications:
- Large-scale dr ug response screening and cytotoxicity testing.
- Circadian rhythm analysis and biological clock research.
- Gene expression profiling for high-throughput research.
The Importance of Real-Time Monitoring in Live Cell Research
Real-time monitoring systems like Cellgraph, CytoWatcherII, Kronos Dio, and Kronos HT provide researchers with the ability to observe cellular behavior and gene expression as it happens, offering a level of insight that traditional endpoint assays cannot match. These systems empower scientists to:
- Track dynamic cellular changes over time.
- Assess cellular responses to treatments or environmental changes.
- Investigate complex processes like cell signaling, genetic regulation, and drug efficacy in real-time.
These capabilities are especially crucial in single-cell analytics, where understanding the behavior of individual cells can reveal valuable insights into cellular diversity, disease mechanisms, and therapeutic responses.
Why Choose Our Live Cell Research Systems?
- Precision and Sensitivity: Our systems are built to provide accurate, high-resolution data that you can trust for your most critical research.
- Ease of Use: Designed with user-friendly interfaces, these systems are accessible for both novice and experienced researchers.
- High Throughput: Systems like the Kronos HT are designed to process large volumes of data efficiently, saving time and increasing productivity.
- Real-Time Monitoring: The ability to track cellular processes as they occur provides deeper insights into the biology of live cells.
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