Blood DNA Extraction Kits: High-Quality Nucleic Acid Isolation for PCR, qPCR & HLA Typing (61E Series)
In modern molecular biology and clinical diagnostics, reliable DNA extraction from blood samples is a critical first step. The 61E Blood DNA Extraction Series provides a fast, automated, and highly efficient solution for isolating high-purity genomic DNA suitable for advanced downstream applications.
Designed for both research and diagnostic laboratories, this system ensures consistent DNA yield, integrity, and purity, making it ideal for sensitive techniques such as PCR, qPCR, HLA typing, and DNA sequencing.
What is the 61E Blood DNA Extraction Kit?
The 61E series is an advanced automated nucleic acid extraction system optimized for whole blood samples. It uses magnetic bead-based technology to efficiently isolate DNA while removing inhibitors that can affect downstream analysis.
✅ Key Features
- High DNA yield and purity
- Compatible with fresh, refrigerated, and frozen blood samples
- Automated workflow (reduced hands-on time)
- Removal of PCR inhibitors
- Reproducible and consistent results
High-Quality DNA for Downstream Applications
The extracted DNA is specifically optimized for:
- PCR (Polymerase Chain Reaction)
- qPCR (Quantitative PCR)
- HLA typing (transplant compatibility testing)
- Next-Generation Sequencing (NGS)
- Genetic mutation analysis
Figure . Nanodrop analysis of the performance and quality of 61E series kit for extracting blood DNA on aelstrom4810. (A) DNA concentration; (B) OD260/280; (C) OD260/230. Fresh blood; Fridge: refrigerated blood; Frozen: frozen blood.
👉 This ensures reliable amplification and accurate results in critical workflows.
DNA Quality & Purity Performance
The 61E series demonstrates excellent performance in nucleic acid quality analysis:
🔬 Nanodrop Analysis
- Optimal DNA concentration yield
- Ideal OD260/280 ratio (~1.8) → indicates protein-free DNA
- Stable OD260/230 ratio → low contamination
These parameters confirm that the extracted DNA is pure and suitable for sensitive molecular assays.
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Agarose gel electrophoresis results show:
- High molecular weight DNA
- Minimal degradation
- Strong, consistent band patterns
Agarose gel electrophoresis of DNA extracted using the TANBead 61E series kit on Maelstrom 4810. The results demonstrate high nucleic acid integrity across different sample conditions. Fresh: fresh blood; Fridge: refrigerated blood; Frozen: frozen blood.
👉 This confirms that the DNA remains intact and suitable for long-range PCR and sequencing applications.
HLA Typing Performance
The 61E extraction kit has been validated for HLA-A, HLA-B, and HLA-C amplification.
✔️ Key Outcomes:
- Clear and strong PCR bands
- Consistent amplification across samples
- High reproducibility
Amplification of HLA-A, HLA-B, and HLA-C from the eluted DNA products. Gel electrophoresis images show consistent fragment size and stable performance for long-range PCR products. A: 61E series; B: T-brand; C: Q-brand.
👉 This makes it highly suitable for:
- Transplantation matching
- Immunogenetics research
- Clinical diagnostics
Applications in Research & Diagnostics
This kit is widely used in:
- 🧬 Genetic research
-
🏥 Clinical diagnostics
- 🧫 Oncology studies
- 🧑⚕️ Transplant compatibility testing (HLA typing)
🚀 Advantages of the 61E Series
- Fast and automated workflow
- High reproducibility
- Compatible with multiple sample conditions
- Optimized for downstream molecular applications
- Reliable performance for clinical and research use
